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CST - Akt Antibody - Cell Signaling Technology (CST): Antibodies, Reagents, Proteomics, Kit Add formaldehyde to obtain a final concentration of 4%. Fix for 10 min at 37 C. Chill tubes on ice for 1 min. For extracellular staining with antibodies that do not require permeabilization, proceed to immunostaining (Section D) or store cells in PBS with
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